This product is intended for laboratory research use only. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedĬomplete growth medium supplemented with 5% (v/v) DMSO ( ATCC 4-X) Allow the flask to sit at room temperature (or at 37☌) until the cells detach.Īdd fresh culture medium, aspirate and dispense into new culture flasks. Remove the solution and add an additional 1 to 2 mL of Trypsin-EDTA solution. Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Discard the supernatant and resuspend the cells with fresh growth medium at the dilution ratio recommended in the specific batch information. If it is desired that the cryoprotective agent be removed immediately, or that a more concentrated cell suspension be obtained, centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. A 5% CO 2 in air atmosphere is recommended if using the medium described on this product sheet. Incubate the culture at 37☌ in a suitable incubator.
It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
It is important to avoid excessive alkalinity of the medium during recovery of the cells.